Vertical Electrophoresis Tank

The vertical electrophoresis tank is acrylic and consists of an upper buffer chamber and a lower buffer chamber. The device has two gel perfusion frames and glass plates that are precisely aligned to prevent gel leakage. A built-in ridge isolates the gel from the air and prevents polymerization reactions from occurring in the gel. The vertical electrophoresis tank is designed for efficient separation and analysis of macromolecules, such as DNA, RNA, and proteins, through gel electrophoresis. This model accommodates sample combs for 10 and 15 samples, with the capacity to run 1-2 gels simultaneously. 

Vertical Electrophoresis Tank Feature:

1. Injection molding of high-strength, extremely transparent materials offers superior compressive strength, improved structural stability, and outstanding physical strength and durability.
2. The transparent appearance design is straightforward and elegant, and it uses a unique glue-making rack for dependable and simple glue-making operations.
3. The glass gasket’s integrated design prevents adhesive leaks and guarantees the vertical electrophoresis tank’s sealing capabilities.

Vertical Electrophoresis Tank Specifications:

Advantages of Vertical Electrophoresis Tank:

1. Compared to concave plates, flat plates are less expensive and less prone to damage.
2. Elevated purity platinum electrode significantly enhances both conductivity and resistance to corrosion.
3. The upper cover’s limit function makes sure that the electrode’s polarity won’t be connected incorrectly.

Application of Vertical Electrophoresis Tank:

1. Molecular Biology: Ideal for the separation and analysis of nucleic acids and proteins.
2. Genetic Research: Used for DNA fingerprinting, genotyping, and molecular cloning.
3. Biotechnology: Essential in protein purification, DNA sequencing, and genetic engineering experiments.
4. Clinical Diagnostics: Utilized in the analysis of clinical samples for diagnostics and research.
5. Educational Laboratories: Perfect for teaching basic and advanced electrophoresis techniques in academic institutions.

Details of ET-V01 Electrophoresis Tank:

This meticulous packaging minimizes the risk of damage and makes it easier for users to identify and access each component quickly.

The simple and clear arrangement of accessories ensures that you can easily and smoothly assemble the vertical electrophoresis tank, facilitating a smooth and uninterrupted workflow.

The simplicity in design ensures that even those new to electrophoresis can assemble the tank with minimal instruction.

Principle of Vertical Electrophoresis Tank

Vertical electrophoresis tank is primarily used for sequencing nucleic acids and proteins through vertical gel electrophoresis. This system employs a discontinuous buffer system composed of upper and lower chambers. The upper chamber houses the cathode, while the lower chamber contains the anode. Electrodes in both chambers create the necessary electric field. A thin gel, less than 2 mm thick, is cast between two glass mounting plates, with the top of the gel submerged in the upper buffer chamber and the bottom in the lower buffer chamber. When an electric field is applied, a small amount of buffer migrates from the upper chamber through the gel to the lower chamber. Since the buffer moves exclusively through the gel, the voltage gradient can be precisely controlled throughout the experiment.

Vertical electrophoresis tanks enable researchers to achieve more efficient separations and higher resolution. Unlike horizontal electrophoresis, vertical systems typically utilize polyacrylamide gels with smaller pore sizes (approximately 10-200 nm). Polyacrylamide gels are unsuitable for horizontal gel electrophoresis because they must interact with ambient oxygen, and exposure to oxygen inhibits acrylamide polymerization, disrupting gel formation. In vertical electrophoresis, the buffer migrates only through the gel, preventing exposure to oxygen in the individual compartments. Additionally, the acrylamide concentration regulates the gel’s pore size and cross-linking. As the total acrylamide concentration increases, the pore size decreases. The amount of crosslinking agent is determined relative to the total acrylamide concentration. Pore size exhibits a parabolic relationship with crosslinking; it is largest at high and low levels of crosslinking and smallest at 4% crosslinking.

 

WHY SCITEK

1. 24 Months Limited Warranty
2. CE & FDA Standards
3. 1-2 Days Handling and 7-30 Days Shipping